The human IL8 coding sequence was insert into mouse ROSA26 gene site in B-hIL8 MC38 cells. Human IL8 is highly expressed in B-hIL8 MC38 cells.

Gene targeting strategy for B-hIL8 MC38 cells. The human IL8 CDS was insert into mouse ROSA26 gene site.

B-hIL8 MC38 cells were subcutaneously transplanted into C57BL/6 mice (n=5), and on 31 days post inoculation, tumor cells were harvested and assessed for human IL8 expression by ELISA. As shown, human IL8 was highly expressed on the supernatant of tumor cells. Therefore, B-hIL8 MC38 cells can be used for in vivo efficacy studies of novel IL8 therapeutics.

Subcutaneous homograft tumor growth of B-hIL8 MC38. B-hIL8 MC38 cells (5x10⁵) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into C57BL/6N mice (female, 8-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hIL8 MC38 were able to establish tumors in vivo and can be used for efficacy studies.