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      B-Luc-EGFP SU-DHL-1

      • 322063

      B-Luc-EGFP SU-DHL-1

      Product nameB-Luc-EGFP SU-DHL-1
      Catalog number322063
      AliasesNA
      TissueLymphoma
      DiseaseLarge cell lymphoma; ddiffusehistiocytic lymphoma
      SpeciesHuman
      ApplicationB-Luc-EGFP SU-DHL-1 cell line can be used to evalsuate anti-cancer drugs in large cell lymphoma or ddiffuse histiocytic lymphoma in vivo

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      • Description
      • Targeting strategy
      • Phenotypic analysis
      • Tumorigenicity

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          Description

          This B-Luc-EGFP SU-DHL-1 cell line expresses firefly luciferase as a marker of SU-DHL-1 cells. Fluorescence and EGFP could be detected in B-Luc-EGFP SU-DHL-1 cells.

          Targeting strategy

          Gene targeting strategy for B-Luc-EGFP SU-DHL-1 cells. The CMV promoter and luciferase-EGFP were inserted into the targeting vector used in the lentivirus system.

          In vitro luciferase activity of B-Luc-EGFP SU-DHL-1 cells

          Luminescence signal intensity of B-Luc-EGFP SU-DHL-1 cells. Luminescence intensity was measured using the Bright-GloTM luciferase Assay. B-Luc-EGFP SU-DHL-1 cells have a strong luminescence signal. EGFP was detected in B-Luc-EGFP SU-DHL-1 cells. The 1-D10 clone of B-Luc-EGFP SU-DHL-1 cells can be used in vivo experiments.

          Quantitative imaging data & body weight changes

          B-Luc-EGFP SU-DHL-1 cells (5x106) were injected into the tail vein of wild-type B-NDG mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-Luc-EGFP SU-DHL-1 cells can be used for in vivo efficacy evalsuation.

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