• The full coding sequences of Luciferase gene, EGFP gene and LacZ gene that is conditional driven by CAG promoter are inserted into mouse Hipp11 (H11) locus in B-H11-CAG-Luc flox mice.
• B-H11-CAG-Luc flox mice were bred with Cd4-Cre transgenic mice, and luciferase expression was detected in the spleen, lymph nodes, and blood.
• B-H11-CAG-Luc flox mice enabled the generation of conditional Luc-overexpressing reporter mice, utilizing the luciferase detection system to track specific cell populations or RNA interference-mediated gene silencing in mice .

Gene targeting strategy for B-H11-CAG-Luc flox mice . The full coding sequences of Luciferase gene, EGFP (enhanced green fluorescent protein) gene and LacZ gene that is conditional driven by CAG promoter are inserted into mouse Hipp11 (H11) locus in B-H11-CAG-Luc flox mice.

Mice were imaged 10min after i.p. injection of 150mg/kg D-Lucifenrin potassium salt using IVIS Lumina LT Inst Series IIIimaging system.The mice in group G2 exhibited significant bioluminescence.The spleens, lymph nodes and blood of mice were respectively imaged. The G2 group and G4 group mice showed significant bioluminescence in the spleen and lymph nodes, and the G2 group mice also exhibited obvious bioluminescence in the blood.