Strain specific IL1RAcP expression analysis in homozygous B-hIL1RAcP mice by flow cytometry. Macrophages and monocytes were collected from wild type and homozygous B-hIL1RAcP mice (H/H) and analyzed by flow cytometry with species-specific anti-IL1RAcP antibody. Human IL1RAcP were exclusively detectable in homozygous B-hIL1RAcP mice but not in wild type mice.

Splenocytes were collected from wild-type mice and homozygous B-hIL1RAcP mice and stimulated with IL1/IL33/IL36 protein. The expression of cytokines in splenocytes culture medium was detected after 48 hours. The results showed that murine IL1/IL33/IL36 protein effectively activated splenocytes in wild-type mice and triggered the secretion of related cytokines. Murine IL1B and murine IL33 proteins could effectively activate splenocytes in B-hIL1RACP mice and trigger the secretion of related cytokines, while murine IL36 protein could not effectively activate B-hIL1RACP mice splenocytes.

Note: This experiment was performed by the client using B-hIL1RACP mice. All the other materials were provided by the client.

Effects of Anti-IL1RAcP antibody on immune cell infiltration in peritoneal lavage fluid in a MSU-induced peritonitis model using B-hIL1RAcP mice. (A) Modeling paradigm of MSU-induced peritonitis model in B-hIL1RAcP mice. (B) Quantification of mCD45⁺ cells in peritoneal lavage fluid across groups. (C) Total neutrophil counts in peritoneal lavage fluid. (D) Percentage of neutrophils within the mCD45⁺ population, demonstrating that the number of neutrophils was significantly inhibited by CAN10 (in house) and ALM27134 (in house) and almost returned to the level of the control group. Values are expressed as mean ± SEM.